Recombinant Human IL-21 Protein
Catalugue Number: GW-TL509-0050
GW-TL509-0100
GW-TL509-1000（Customize）
Filling Volume: 50μg、100μg、1mg
Stock: In Stock
Product Information
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Cytokines-Recombinant Human IL-21 Protein |
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| Product Name | Recombinant Human IL-21 Protein |
| Expression Host | CHO cells |
| QC Testing Purity | >95% as determined by SDS-PAGE |
| Activity | Measured by its ability of inducing human natural killer lymphoma NK-92 cells to secrete interferon-γ. The expected ED₅₀ is ≤50ng/ml. |
| Endotoxin | <0.01 EU per μg of the protein as determined by the LAL method. |
| Molecular Mass | The recombinant human IL-21 predicts a molecular mass of 41.9 kD. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
| Stability & Storage |
Lyophilized preparation can be stored at -20 ℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
| Background | IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells; the stimulation of IgG production in conjunction with IL-4; and the induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. |
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